Table 1.
Mature dendritic cells were cultured with and without cocaine (1 µM) for 48 hr (n = 4 independent experiments).
| Spot # | Protein name | Gene accession number |
% change | p value | Theoretical mass |
Theoretical pI |
Biological function |
|---|---|---|---|---|---|---|---|
| 916 | programmed cell death 6 interacting protein (AIP1) |
gi|34866400| | 25 ↑ | 0.001 | 70545.76 | 7.45 | regulation of apoptosis actin binding protein |
| 1738 | cathepsin D preproprotein | gi|4503143| | 19 ↑ | 0.01 | 44552.22 | 6.10 | aspartic protease |
| 1766 | enolase | gi|4503571| | 16 ↑ | 0.05 | 47168.96 | 7.01 | glycolysis |
| 1766 | non-neural alpha enolase | gi|22096350| | 16 ↑ | 0.05 | 47200.91 | 5.84 | glycolysis |
| 1766 | nuclear factor NF-kappa-B p105 subunit |
gi|21542418| | 16 ↑ | 0.05 | 105356.00 | 5.20 | transcription factor |
| 2274 | D-2-hydroxy-acid dehydrogenase |
gi|11399466| | 28 ↑ | 0.01 | 27312.44 | 9.10 | oxidoreductase |
| 2274 | hypothetical protein DKFZp564C047.1 |
gi|11360180| | 28 ↑ | 0.01 | 58140.27 | 4.94 | unknown |
| 2274 | heterogeneous nuclear ribonucleoprotein H3 |
gi|14141157| | 28 ↑ | 0.01 | 36926.49 | 6.37 | RNA binding protein |
| 2929 | phosphoglycerate mutase 1 | gi|4505753| | 13 ↑ | 0.01 | 28803.93 | 6.67 | glycolysis/gluconeogenesis |
| 2969 | aldolase A (AA) | gi|229674| | 16 ↑ | 0.007 | 39288.83 | 8.39 | glycolysis |
| 2969 | aldolase C, fructose- bisphosphate |
gi|4885063| | 16 ↑ | 0.007 | 39455.87 | 6.41 | glycolysis |
| 3015 | tumor protein | gi|4507669| | 40 ↑ | 0.003 | 19595.34 | 4.84 | unknown |
| 865 | heat shock 90kDa protein (HSP90) |
gi|13129150| | 11 ↓ | 0.001 | 84659.71 | 4.94 | molecular chaperone |
| 1122 | heat shock 70 kDa (HSP70) |
gi|662802| | 16 ↓ | 0.004 | 71194.37 | 5.36 | molecular chaperone |
| 1122 | L-plastin | gi|4504965| | 16 ↓ | 0.004 | 70289.33 | 5.20 | actin-binding protein |
| 1673 | heterogeneous nuclear ribonucleoprotein H2 |
gi|9624998| | 11 ↓ | 0.005 | 49263.57 | 5.89 | ribonucleoprotein |
| 1673 | tryptophan–tRNA ligase | gi|135191| | 11 ↓ | 0.005 | 53165.42 | 5.83 | ligase activity |
| 2007 | protein phosphatase 1 regulatory inhibitor subunit 8(NIPP1) |
gi|13699256| | 22 ↓ | 0.002 | 38478.79 | 6.87 | RNA binding protein |
| 2007 | phosphoglycerate kinase 1 | gi|4505763| | 22 ↓ | 0.002 | 44614.69 | 8.30 | glycolysis |
| 2288 | glyceraldehyde-3- phosphate dehydrogenase |
gi|7669492| | 16 ↓ | 0.05 | 36053.21 | 8.57 | glycolysis/gluconeogenesis |
| 2595 | carbonyl reductase 1 | gi|4502599| | 27 ↓ | 0.004 | 30374.94 | 8.55 | KEGG pathway: |
| 2964 | similar to high mobility group box 1 |
gi|27479871| | 24 ↓ | 0.007 | 24665.60 | 5.92 | nonhistone chromosomal protein |
Listed are statistically significant, differentially expressed proteins (Student’s t-test) that were identified by HPLC-MS/MS (all proteins had an expect score >10). Theoretical mass and pI were calculated using: http://au.expasy.org/tools/pi_tool.html.