Table 2.
Mature dendritic cells were cultured with and without methamphetamine (100 µM) for 24 hr (n = 4 independent experiments).
| Spot # | Protein name | Gene accession number |
% change | p value | Theoretical mass |
Theoretical pI |
Biological function |
|---|---|---|---|---|---|---|---|
| 795 | programmed cell death 6 interacting protein (AIP1) |
gi|31076784| | 61 ↑ | 0.001 | 44060.92 | 8.68 | signal transduction |
| 1436 | heat shock protein 70kDa, protein 8 (HSPA8) |
gi|5729877| | 21 ↑ | 0.028 | 70898.09 | 5.37 | molecular chaperone |
| 1436 | L-plastin | gi|4504965| | 21 ↑ | 0.028 | 70289.33 | 5.20 | calcium and actin binding |
| 1436 | laminin B1 | gi|5031877| | 21 ↑ | 0.028 | 66408.34 | 5.11 | protein binding |
| 1679 | calreticulin | gi|4757900| | 52 ↑ | 0.004 | 48141.56 | 4.29 | ER protein |
| 2175 | calreticulin | gi|4757900| | 67 ↑ | 0.003 | 48141.56 | 4.29 | ER protein |
| 2003 | nuclease sensitive element binding protein 1 (YB-1) |
gi|423015| | 70 ↑ | 0.023 | 34541.60 | 7.78 | DNA, RNA binding, Transcription factor |
| 2158 | mitogen-activated protein kinase kinase 2 (MEK1) |
gi|5579478| | 43 ↑ | 0.011 | 43439.01 | 6.18 | serine/threonine/ tyrosine kinase activity |
| 2158 | mitogen-activated protein kinase kinase 1 (MEK2) |
gi|13489054| | 43 ↑ | 0.011 | 44424.13 | 6.12 | serine/threonine/ tyrosine kinase activity |
| 2158 | stress induced phosphoprotein 1 |
gi|5803181| | 43 ↑ | 0.011 | 62639.26 | 6.40 | protein binding |
| 2176 | membrane-organizing extension spike protein (moesin) |
gi|4505257| | 36 ↑ | 0.008 | 67820.04 | 6.08 | cytoskeletal protein binding |
| 3028 | hydroxyacyl-Coenzyme A dehydrogenase |
gi|4758504| | 59 ↑ | 0.044 | 26923.08 | 7.65 | catalytic activity |
| 3055 | cathepsin S | gi|23200070| | 51 ↑ | 0.034 | 23947.91 | 7.66 | lysosomal cysteine proteinase |
| 1899 | lymphocyte-specific protein 1 |
gi|10880979| | 47 ↓ | 0.017 | 37191.59 | 4.69 | signal transduction |
| 2172 | pyruvate kinase | gi|4505839| | 39 ↓ | 0.026 | 57913.85 | 7.95 | kinase activity |
| 2200 | pyruvate kinase | gi|4505839| | 35 ↓ | 0.004 | 57913.85 | 7.95 | kinase activity |
| 2173 | heat shock 70kDa, protein 5 (HSPA5) |
gi|87528| | 32 ↓ | 0.037 | 72115.65 | 5.03 | molecular chaperone |
| 2195 | adenosine kinase | gi|7448823| | 23 ↓ | 0.036 | 37511.82 | 6.09 | phosphotransferase |
| 2377 | 3(2),5-bisphosphate nucleotidase |
gi|25282455| | 33 ↓ | 0.008 | 33174.03 | 5.58 | phosphatase activity |
| 2411 | heterogeneous nuclear ribonucleoprotein C (hRNPC) |
gi|4758544| | 26 ↓ | 0.003 | 31966.24 | 5.10 | RNA binding |
| 2411 | laminin receptor 1 | gi|9845502| | 58 ↓ | 0.003 | 32854.08 | 4.79 | receptor activity |
| 2411 | nucleophosmin (B23) | gi|112079| | 58 ↓ | 0.003 | 28385.22 | 4.54 | molecular chaperone |
| 2553 | 26S proteasome- associated pad1 homolog |
gi|34854852| | 26 ↓ | 0.042 | 41125.53 | 6.94 | unknown |
| 2556 | F-actin capping protein alpha-1 subunit |
gi|5453597| | 24 ↓ | 0.03 | 32922.77 | 5.45 | actin binding |
| 2569 | lactate dehydrogenase | gi|13786847| | 32 ↓ | 0.002 | 36507.30 | 5.72 | oxidoreductase activity |
| 2643 | gp96 | gi|6755863| | 73 ↓ | 0.041 | 92475.77 | 4.74 | molecular chaperone |
| 2693 | EF hand domain family member D2 |
gi|20149675| | 76 ↓ | 0.009 | 26697.28 | 5.15 | calcium ion binding |
| 2717 | alpha-SNAP | gi|4505329| | 54 ↓ | 0.009 | 33246.75 | 5.23 | intracellular transporter |
| 2728 | ribosomal protein P0 | gi|4506667| | 67 ↓ | 0.004 | 34273.51 | 5.72 | RNA and protein binding |
| 2770 | P32 A Doughnut-Shaped Acidic Mitochondrial Matrix Protein |
gi|4930073| | 86 ↓ | 0.021 | 23801.17 | 4.32 | Unknown |
Listed are statistically significant, differentially expressed proteins (Student’s t-test) that were identified using HPLC-MS/MS (all proteins had an expect score >10). Theoretical mass and pI were calculated using: http://au.expasy.org/tools/pi_tool.html.