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. 1998 Dec 22;95(26):15826–15830. doi: 10.1073/pnas.95.26.15826

Figure 2.

Figure 2

Spectrophotometric and Western blot analysis of recombinant Adiantum PHY3. (A) Zinc blot (lanes 1 and 2) and Strep-tag Western blot (lanes 3 and 4) analyses. Each lane contained 300 ng of Adiantum PHY3 extract to which phycocyanobilin (PCB) was added (lanes 2 and 4) or omitted (lanes 1 and 3) and then resolved on 10% SDS/PAGE gels and transblotted to poly(vinylidene difluoride) membrane. Lanes 1 and 2 show zinc-treated membrane visualized by fluorescence using a Molecular Dynamics Storm imager. Lanes 3 and 4 are the same protein blot probed with streptavidin-alkaline phosphatase conjugate. Full-length of Adiantum PHY3 PCB adducts (indicated by arrowhead) show fluorescence on zinc blot only when PCB was added (lane 2). Marker sizes are shown on the left in kDa. (B) Phytochrome-difference spectrum of Adiantum PHY3 after incubation with PCB used in A.