TABLE 2.
Oligonucleotides used in this study
| Primer | Oligonucleotide sequence (5′→3′)a |
|---|---|
| chpA22 | GGACTAGTAGATGGCGAGCGAGATGC |
| chpA25 | GAGCTCCTCGCGCAGCGC |
| chpA26.1 | GGCGCGCAACGGCCATTG |
| chpA29 | GGACTAGTCGCAGCGTGCGGCCAGAT |
| chpA-HisKn-F | CTCCTCACCCTCTCCGCCGCCGCCGCCGGCATCCGCCTC |
| chpA-HisKn-R | GAGGCGGATGCCGGCGGCGGCGGCGGAGAGGGTGAGGAG |
| fliC1 | GGACTAGTACCGTTTCGTGGTCTCGA |
| fliC2 | TTAGCGCAGCAGAAGCTTAAGGGCCATGGTGATTTCCTCCAAAGG |
| fliC3 | ATGGCCCTTAAGCTTCTGCTGCGCTAAGCCCGGGAACGGTCACTC |
| fliC4 | GGACTAGTATTGATGGCGTCACGCAT |
| pilA1 | CCATGGATGCCTAACCTCACCCTTGC |
| pilA1(Spe) | ACTAGTATGCCTAACCTCACCCTTGC |
| pilA2 | GAATTCTAAGGTGATCGAAGGTGGCTTGTTTAG |
| pilA3 | GAATTCCATGAATATCTCCATTGATATG |
| pilA4 | TCTAGATTGATCCGTCCGTCCTGCGG |
| pilA4(Spe) | ACTAGTTTGATCCGTCCGTCCTGCGG |
| pilA-Reporter-F | CGCGGATCCGAATCTCTCCGTTGATTA |
| pilA-Reporter-R | CCGCTCGAGGTGTTGGCGGACCAGCTT |
| pilB1 | GCACTAGTATCAACGAGGGCACCCTGCGC |
| pilB2 | TTAATCCTTGGTAAGCTTGTCGTTCATGGGGAAGGAATCGCAGAAGGG |
| pilB3 | ATGAACGACAAGCTTACCAAGGATTAATCCATGGCGGACAAAGCGTTA |
| pilB4 | GCACTAGTTGGATCGCCATGTTGGGAAAG |
| pilG1 | CGGGGTACCCTCAGCGGCTGGGCCACGCCGCG |
| pilG1(Spe) | GCACTAGTCTCAGCGGCTGGGCCACGCCGCG |
| pilG2 | AAGCTTTTGCTGTTCCATGTTCGCCC |
| pilG3 | AAGCTTAAGGGGCGCATCGTCGGCTC |
| pilG4 | TCTAGAATGAAGGGTTGCAGTGCCGC |
| pilG4(Spe) | GCACTAGTATGAAGGGTTGCAGTGCCGC |
| pilG5 | TCAGTGGTGGTGGTGGTGGTGGGAAACGGCGTCCACCGGGGT |
| pilG6 | ACCCCGGTGGACGCCGTTTCCCACCACCACCACCACCACTGA |
| pilG-D58A-F | AACATCATTTTCGTCGCCATCATGATGCCGCGC |
| pilG-D58A-R | GCGCGGCATCATGATGGCGACGAAAATGATGTT |
| pilH1 | GGTACCCGTTATCGAAGGGCGGGTCC |
| pilH1(Spe) | GCACTAGTCGTTATCGAAGGGCGGGTCC |
| pilH4 | TCTAGAGCTTTCTCGAAGTCGTTGCG |
| pilH4(Spe) | GCACTAGTGCTTTCTCGAAGTCGTTGCG |
| pilH15 | TCAGTGGTGGTGGTGGTGGTGGCCCGCCAGCACCGCATTG |
| pilH16 | CAATGCGGTGCTGGCGGGCCACCACCACCACCACCACTGA |
| pilH-D52A-F | GACGTGGTCCTGATGGCCATCGTCATGCCCGGC |
| pilH-D52A-R | GCCGGGCATGACGATGGCCATCAGGACCACGTC |
| pilR1 | GGACTAGTCTGCAGCGCATGCGCACC |
| pilR2 | TCAGTCGATGCCAAGCTTTCGGCTCATGCGTGCGGCTTCCGTCAG |
| pilR3 | ATGAGCCGAAAGCTTGGCATCGACTGAAAGTGAAAAGGCCTGTCC |
| pilR4 | GGACTAGTATCGTCTCGCCGCTCTAT |
| pilT1 | GGACTAGTTGGCACACGGCTTTCATGG |
| pilT1(new) | GGACTAGTAACTCGCCGGCGATCTTC |
| pilT4 | GGACTAGTTGTAGAGCTGGTACAGCGC |
| pilT5 | TCAGAAGTTTTCAAGCTTAATATCCATGGGACTCCCCAATTACAAGC |
| pilT6 | ATGGATATTAAGCTTGAAAACTTCTGATCCTGGCGCCGATCCGC |
| pilT-Trunc2 | CAGGTAATCGAGTCTAGTCTAGACTAGGGGACTCCCCAATTACAAGCAAGCAGG |
| pilT-Trunc3 | GGGAGTCCCCTAGTCTAGACTAGACTCGATTACCTGAACAACACCAAGTACCAC |
| pilT-Trunc4 | GGACTAGTGAAGTTGCACTCATGGTT |
| pilU1 | GGACTAGTAACACAAGCAGGTGCATGC |
| pilU4 | GGACTAGTAAGGTGAAGAACCAGATCG |
| pilU5 | TCAGCGGAAGCGTCTAGAGAATTCCATGATGTTCTCGCTCACTCAGG |
| pilU6 | ATGGAATTCTCTAGACGCTTCCGCTGAACCGTCTTCAGTAGGCCAGC |
| pilTpilU1 | TCAGCGGAAGCGAATATCCATGGGACTCCCCAATTACAAGC |
| pilTpilU2 | ATGGATATTCGCTTCCGCTGAACCGTCTTCAGTAGGCCAGC |
| pilU-CTX-Spe-F | GGACTAGTGACCGGGTGGTCGACGTGTTC |
| pilU-CTX-Hind-R | CCCAAGCTTTCAGCGGAAGCGCCGGCCGGC |
a
Restriction endonuclease sequences are underlined.