TABLE 3.

Sensitivity of various strains to infection by the pilus-specific phage PO4

Strain	Strain sensitivity (avg PFU/ml ± SD [109])a
PAO1	3.96 ± 0.53
PAO1ΔchpA	3.30 ± 0.70
PAO1ΔchpA(comp)	3.75 ± 0.79
PAO1ΔpilG	2.85 ± 0.72
PAO1ΔpilG(comp)	4.52 ± 0.66
PAO1ΔpilB	0.00 ± 0.00*
PAO1ΔpilB(comp)	3.92 ± 0.32
PAO1ΔpilA	0.00 ± 0.00*
PAO1ΔpilA(comp)	4.12 ± 0.56
PAO1ΔfliC	3.92 ± 0.56
PAO1ΔfliC(comp)	5.41 ± 0.14
PAO1ΔpilH	2.96 ± 0.58†
PAO1ΔpilH(comp)	3.48 ± 0.45
PAO1ΔpilTCTX-pilU	0.00 ± 0.00*
PAO1ΔpilT(comp)	3.39 ± 0.17
PAO1ΔpilU	2.88 ± 0.33
PAO1ΔpilU(comp)	4.11 ± 0.48
PAO1ΔchpAΔpilH	4.00 ± 0.45†
PAO1ΔchpAΔpilTCTX-pilU	0.00 ± 0.00*
PAO1ΔchpAΔpilU	1.85 ± 0.30
PAO1ΔchpAΔpilTΔpilU	0.00 ± 0.00*
PAO1ΔpilGΔpilH	3.93 ± 0.72†
PAO1ΔpilGΔpilTCTX-pilU	0.00 ± 0.00*
PAO1ΔpilGΔpilU	3.07 ± 0.78
PAO1ΔpilGΔpilTΔpilU	0.00 ± 0.00*
PAO1ΔpilBΔpilH	0.00 ± 0.00*
PAO1ΔpilBΔpilTCTX-pilU	0.00 ± 0.00*
PAO1ΔpilBΔpilU	0.00 ± 0.00*
PAO1ΔpilBΔpilTΔpilU	0.00 ± 0.00*
PAO1ΔpilHΔpilTCTX-pilU	0.00 ± 0.00*
PAO1ΔpilTΔpilU	0.00 ± 0.00*
PAO1ΔpilHΔpilU	2.87 ± 0.83†
PAO1ΔpilHΔpilTΔpilU	0.00 ± 0.00*
PAO1chpA-FLAG	3.45 ± 0.63
PAO1ΔchpA(AAA)-FLAG	3.28 ± 0.63
PAO1pilG-His	3.17 ± 0.29
PAO1pilG(D58A)-His	2.99 ± 0.39
PAO1pilH-His	3.31 ± 0.34
PAO1pilH(D52A)-His	4.23 ± 0.23†

^a*, No plaques were detectable even when strains were infected with 10⁹ phage. †, The ΔpilH plaque morphology was distinguished by large zones of seemingly directional lysis and poorly defined plaques.