FIG. 5.
The rdar morphotypes of Salmonella ATCCcrl harboring different plasmids. The Δcrl mutant harboring the vector pACYC184, the wild-type crl gene on pACcrl-1, and mutated pACcrl-1 derivatives (Table 1) were grown for 5 days on CR plates at 28°C. Plasmids are as follows: spot 1, pACYC184; spot 2, pACcrl-1; spot 3, pACcrlY22A; spot 4, pACcrlF35A; spot 5, pACcrlC37A; spot 6, pACcrlC41A; spot 7, pACcrlF53A; spot 8, pACcrlW56A; spot 9, pACcrlG74A; spot 10, pACcrlG80A; spot 11, pACcrlW82A; spot 12, pACcrlF103A; spot 13, pACcrlC28A; spot 14, pACcrlC28-37-41. Complementation of the crl mutation was observed with pACcrl-1 and its derivatives containing mutations F35A, C28A, C37A, C41A, G74A, G80A, and F103A.