TABLE 3.
Promoter activity of ropB, ropB2, and ropB3 in VF39SM under various growth conditions
| Plasmid | gusA reporter fusion |
gusA activitya |
|||
|---|---|---|---|---|---|
| Strains grown in TY | Strains grown in VMM | Strains grown in VMM + 1 mM EDTA | Bacteroids | ||
| pDF4 | ropB::gusA | 5.37 × 103 ± 1.40 × 103 | 690 ± 279* | 1.61 × 103 ± 47.8 | 233 ± 62.4* |
| pDF40 | ropB2::gusA | 240 ± 27.8 | 194 ± 32.3 | 139 ± 62.6 | 116 ± 30.0 |
| pDF41 | ropB3::gusA | 417 ± 61.9 | 199 ± 30.0 | 307 ± 53.9 | 114 ± 11.9 |
a
Data are expressed as the means ± standard deviations from at least three assays, and values are expressed as Miller units (27). The average background level of gusA activity for TY-grown cells carrying pFus1 is approximately 250 ± 27.2 Miller units. An asterisk indicates a significantly different mean expression compared to expression in TY for each respective gene (t test; P of <0.005).