FIG. 3.

Coexpression experiment results. Extracts from E. coli cells overproducing CotC and CotU (lane 1), CotC, CotU, and CotE (lane 2) and CotE (lane 3) were fractionated on 15% polyacrylamide gels, electrotransferred to membranes, and reacted with anti-CotC (A) or anti-CotE (B) antibody. Results identical to those of panel A were obtained with anti-CotU antibody (not shown). Panel C shows the position of the three peptides identified as diagnostic of CotC or CotU by the MALDI-TOF experiment results shown in Table 2.