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. 2009 Dec 18;192(4):949–954. doi: 10.1128/JB.01408-09

TABLE 2.

Molecular masses obtained by MALDI-TOF mass spectrometry of tryptic digests of bands CotC, CotU, and CotC-CotUb

Peptide Sequence positionsa Theoretical Mr (M+H)+ Experimental Mr (M+H)+ of band:
CotC CotU CotC-CotC CotC-CotU
1 40-54 1,869.83 1,869.78 1,869.78
2 45-66 2,964.42 2,964.43 2,964.46 2,964.46
3 68-86 2,539.04 2,539.01 2,538.91
a

Refers to the amino acid sequence of CotC (peptide 2) and CotU (peptides 1 and 3) as reported in Fig. 3C.

b

Theoretical and experimental masses together with peptide sequence positions are reported (Da).