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. 2009 Dec 18;192(4):984–993. doi: 10.1128/JB.01169-09

TABLE 1.

Primers used in the study

Primer Sequence (5′ to 3′)a Description (reference)
GLT P1 GCAACAACTCAAACTCACGGTG glnQHMP mutagenesis
GLT P2 GGCGCGCCACCAAGTAAAACAACAACCTGTCC glnQHMP mutagenesis
GLT P3 GGCCGGCCAAAGTGGGAACAAGCAACGG glnQHMP mutagenesis
GLT P4 TCAATGAGCCATCAAGCGAC glnQHMP mutagenesis
ERM For GGCGCGCCCCGGGCCCAAAATTTGTTTGAT Erythromycin cassette (27)
ERM Rev GGCCGGCCAGTCGGCAGCGACTCATAGAAT Erythromycin cassette (27)
16S RT For CTTACCAGGTCTTGACATCCCG 16S rRNA qRT-PCR
16S RT Rev ACCCAACATCTCACGACACGAG 16S rRNA qRT-PCR
1519 RT For GACAGGTTGTTGTTTTACTTG glnQ qRT-PCR
1519 RT Rev GGTCCTTAGTTGAAGCATTGG glnQ qRT-PCR
1520 RT For ATGCTGAGACGGGTAAATACG glnH qRT-PCR
1520 RT Rev GAGGTTCACGAGTTTGAGTCG glnH qRT-PCR
1521 RT For GAAGTCATTCGCTCTGGTATTGAAG glnM qRT-PCR
1521 RT Rev CATTGGTGGCAAGATAGTTCTGATG glnM qRT-PCR
1522 RT For AAATGAAATCCACTCCTGCTGG glnP qRT-PCR
1522 RT Rev CAAGTCCTGCCTCAGTTTGTCC glnP qRT-PCR
1530 RT For GACCGTCAGTGATGGAAATAACG atpD qRT-PCR
1530 RT Rev TCTTCATAGCCGTCTTTTGGAAC atpD qRT-PCR
1531 RT For CTTACAACTTCAGATTTAGCAG atpE qRT-PCR
1531 RT Rev AGAGATTCAGTCCCTATTATC atpE qRT-PCR
1532 RT For CGGCTAAAAGAACACTAAG atpF qRT-PCR
1532 RT Rev CGGTCGTCTAAAAGATAAG atpF qRT-PCR
1534 RT For ACCATACATTTCAGGCTG atpH qRT-PCR
1534 RT Rev TTTTAGCACTTGGGATTG atpH qRT-PCR
a

Restriction sites are underlined: AscI, GGCGCGCC; FseI, GGCCGGCC.