TABLE 1.

Primers used in the study

Primer	Sequence (5′ to 3′)a	Description (reference)
GLT P1	GCAACAACTCAAACTCACGGTG	glnQHMP mutagenesis
GLT P2	GGCGCGCCACCAAGTAAAACAACAACCTGTCC	glnQHMP mutagenesis
GLT P3	GGCCGGCCAAAGTGGGAACAAGCAACGG	glnQHMP mutagenesis
GLT P4	TCAATGAGCCATCAAGCGAC	glnQHMP mutagenesis
ERM For	GGCGCGCCCCGGGCCCAAAATTTGTTTGAT	Erythromycin cassette (27)
ERM Rev	GGCCGGCCAGTCGGCAGCGACTCATAGAAT	Erythromycin cassette (27)
16S RT For	CTTACCAGGTCTTGACATCCCG	16S rRNA qRT-PCR
16S RT Rev	ACCCAACATCTCACGACACGAG	16S rRNA qRT-PCR
1519 RT For	GACAGGTTGTTGTTTTACTTG	glnQ qRT-PCR
1519 RT Rev	GGTCCTTAGTTGAAGCATTGG	glnQ qRT-PCR
1520 RT For	ATGCTGAGACGGGTAAATACG	glnH qRT-PCR
1520 RT Rev	GAGGTTCACGAGTTTGAGTCG	glnH qRT-PCR
1521 RT For	GAAGTCATTCGCTCTGGTATTGAAG	glnM qRT-PCR
1521 RT Rev	CATTGGTGGCAAGATAGTTCTGATG	glnM qRT-PCR
1522 RT For	AAATGAAATCCACTCCTGCTGG	glnP qRT-PCR
1522 RT Rev	CAAGTCCTGCCTCAGTTTGTCC	glnP qRT-PCR
1530 RT For	GACCGTCAGTGATGGAAATAACG	atpD qRT-PCR
1530 RT Rev	TCTTCATAGCCGTCTTTTGGAAC	atpD qRT-PCR
1531 RT For	CTTACAACTTCAGATTTAGCAG	atpE qRT-PCR
1531 RT Rev	AGAGATTCAGTCCCTATTATC	atpE qRT-PCR
1532 RT For	CGGCTAAAAGAACACTAAG	atpF qRT-PCR
1532 RT Rev	CGGTCGTCTAAAAGATAAG	atpF qRT-PCR
1534 RT For	ACCATACATTTCAGGCTG	atpH qRT-PCR
1534 RT Rev	TTTTAGCACTTGGGATTG	atpH qRT-PCR

^aRestriction sites are underlined: AscI, GGCGCGCC; FseI, GGCCGGCC.