TABLE 2.
Primers and plasmids used in the study
| Primer or plasmid | Sequence (5′-3′) or relevant characteristic(s)a,b | Source or reference |
|---|---|---|
| Primers | ||
| CJE0566-Fint | CAAAGTTGTTCGCAAGTTTTAG | |
| CJE0566-Rint | CCTTTTAAGATTTTAATATAAGAG | |
| CJE1441-Fint | CAAAACTGCTCACAAGTTTTGG | |
| CJE1441-Rint | CCTTTTAAAATCTTAGTGTAAGAG | |
| CJE0566-F | GAAATGATTAAAGCAACAAGTTAAAC | |
| CJE0566-R | GTAAGCAAAAAGCACCCCATTC | |
| CJE1441-F | CAAATTGAAAGGGTATGAAAC | |
| CJE1441-R | TGCAATTTCCATTTTTACTCC | |
| CJE0566-Fexp | GATCTTCTAGAAAGGAGAATAAATGAAAAAACTCATAC | |
| CJE0566-Rexp | CCATGGCAATTGTTATATTTTATCACAATC | |
| CJE0566-1441-Fexp | AGATCTTCTAGAAAGGAGAATAAATGAAAAAACTTATAATC | |
| CJE1441-Rexp | CCATGGCAATTGTTAAAAATTGTCACATTTTAC | |
| Pr1492XbaI | AGATCTTCTAGACTCATTTAACGGTTGTCTCC | |
| Pr1492MfeI | ACTAGTCAATTGGCGATGGCCCTG | |
| pWM1007Pr1492-F | GCATATGAAGAGATTTTG | |
| pWM1007-R | CCAACTGGTAATGGTAG | |
| Plasmids | ||
| pCR2.1 | TA cloning vector, Ampr | Invitrogen |
| pCRCJE0566RM1221 | pCR2.1 containing CJE0566 of RM1221 | This study |
| pCRCJE1441RM1221 | pCR2.1 containing CJE1441 of RM1221 | This study |
| pCRCJE056607479 | pCR2.1 containing a CJE0566/CJE1441 homologue of 07479 | This study |
| pWM1007Pr1492 | C. jejuni expression plasmid containing the CJ1492 promoter, Kmr | 36 |
| pWM1007Pr1492CJE0566RM1221 | C. jejuni expression plasmid with CJE0566 of RM1221 | This study |
| pWM1007Pr1492CJE1441RM1221 | C. jejuni expression plasmid with CJE1441 of RM1221 | This study |
| pWM1007Pr1492CJE056607479 | C. jejuni expression plasmid with CJE0566/CJE1441 homologue of 07479 | This study |
| pHipO::cat | C. jejuni suicide vector with cat inserted in hipO, Cmr | 7 |
a
Endonuclease restriction sites introduced into the sequences are underlined.
b
Ribosomal binding sites are shown in bold.