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. 2009 Nov 20;192(4):942–948. doi: 10.1128/JB.01259-09

FIG. 1.

FIG. 1.

Histidine pulldown experiments. SDS-PAGE analysis of the interaction in vitro between purified MarR and GyrA-His. (A) MarR alone was loaded on a nickel column and washed, and any bound proteins were eluted with imidazole. Lanes: S, sample of MarR (5 μg) alone loaded on the nickel column; FT, flowthrough; W1 to W3, washes; E1 to E3, elution with 50, 75, and 125 mM imidazole, respectively. (B) GyrA-His (10 μg; 100 pmol) was loaded on a nickel column followed by MarR (5 μg; 310 pmol). The column was incubated overnight before being washed, and any bound proteins were eluted with imidazole. Lanes: S, sample of GyrA-His loaded on the nickel column; FT, flowthrough; W1 to W3, washes; E1 to E3, elution with 50, 75, and 125 mM imidazole, respectively.