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Effect of GyrA and TktA on MarR DNA binding activity in vitro. Electrophoretic mobility shift assays in phosphate buffer (pH 6.8) of different mixes, as described in Materials and Methods. DNA probe marO (2 pmol), MarR (30 pmol), TktA (120 pmol), and GyrA (50 pmol) in 10 μl (final volume). The 198-base pair target DNA containing both the mar promoter and operator regions (marO) (A and B, lane 1); the GyrA-marO complex (B); the MarR-marO complex (A and B, lane 2).
