TABLE 2.
Protein | Plasmids |
Cell phenotype (X-Gal) | |
---|---|---|---|
pUT18 | pKT25 | ||
Two-hybrid Zip control | Control | Control | White |
Zip | Control | White | |
Control | zip | White | |
Zip | zip | Blue | |
MarR partner | Control | gyrA | White |
marR | Control | White | |
marR | gyrA | Blue | |
MarR mutants with partner | marR Arg77His | gyrA | Blue |
DNA binding | marR Gly116Ser | gyrA | Blue |
Dimerization | marR Ser34Phe/Pro35Ser | gyrA | Blue |
Superrepressor | marR Asp26Asn | gyrA | Blue |
marR Gly95Ser | gyrA | White | |
marR Leu135Phe | gyrA | Blue |
Zip is the 35-codon-long leucine zipper domain of the yeast GNC4 activator used as a positive control of the two-hybrid system. A blue color indicates interaction between proteins specified by the two plasmids (see Materials and Methods). White color indicates no interaction.