TABLE 2.
Two-hybrid interactions between MarR and GyrA identified by LacZ activity (blue colonies)a
| Protein | Plasmids |
Cell phenotype (X-Gal) | |
|---|---|---|---|
| pUT18 | pKT25 | ||
| Two-hybrid Zip control | Control | Control | White |
| Zip | Control | White | |
| Control | zip | White | |
| Zip | zip | Blue | |
| MarR partner | Control | gyrA | White |
| marR | Control | White | |
| marR | gyrA | Blue | |
| MarR mutants with partner | marR Arg77His | gyrA | Blue |
| DNA binding | marR Gly116Ser | gyrA | Blue |
| Dimerization | marR Ser34Phe/Pro35Ser | gyrA | Blue |
| Superrepressor | marR Asp26Asn | gyrA | Blue |
| marR Gly95Ser | gyrA | White | |
| marR Leu135Phe | gyrA | Blue | |
a
Zip is the 35-codon-long leucine zipper domain of the yeast GNC4 activator used as a positive control of the two-hybrid system. A blue color indicates interaction between proteins specified by the two plasmids (see Materials and Methods). White color indicates no interaction.