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. 2009 Dec 11;192(4):1088–1096. doi: 10.1128/JB.01297-09

FIG. 4.

FIG. 4.

Effects of PatA, PatS, and HetN on hetR transcription. (A) RACE results. Lane 1, wild-type strain; lane 2, hetR deletion strain UHM103; lane 3, patA deletion strain UHM101; lane 4, strain 7120PN, which overexpresses hetN from the petE promoter; lane 5, wild-type strain bearing pDR211, which overexpresses patS from the petE promoter. The upper panel shows products from the −728 and −696 tsps, and the lower panel shows products from the −271 and −184 tsps. (B) Northern blot results obtained using RNA isolated after 18 h of growth in BG110. Lane 1, strain 7120PN, which overexpresses hetN from the petE promoter; lane 2, wild type bearing plasmid pDR211, which overexpresses patS from the petE promoter; lane 3, wild type; lane 4, hetR deletion strain UHM103; lane 5, patA deletion stain UHM101. The lower panel shows the results for methylene blue-stained RNA as loading control.