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Growth of El Tor strain N16961 and classical strain O395 in 24-h conditioned medium. V. cholerae El Tor strain N16961 and classical strain O395 were grown separately in LB medium for 24 h. Five milliliters of each culture was centrifuged, washed, and suspended in equal volumes of 24-h culture filtrates of N16961 (circles) or O395 (squares), and the number of CFU was determined at regular intervals.
