FIG. 2.

The effect of c-di-GMP on the conformational structure and promoter binding activity of Clp. (A) EMSA analysis of the impact of c-di-GMP on Clp binding to the engXCA promoter. DIG-labeled promoter fragments were incubated with purified Clp proteins in the presence or absence of nucleotide, as indicated. The final concentration of nucleotide in the reaction mixture was 10 μM or 100 μM. (B and C) Far-UV CD spectra of Clp and the control, glutathione-S-transferase (GST), in the presence or absence of c-di-GMP, as indicated. deg, degrees; com², cm².