FIG. 3.

Modulation of the intracellular level of c-di-GMP changes the expression pattern of engXCA. (A) The effect of rpfG and clp mutations on engXCA transcription in X. campestris pv. campestris (Xcc). (B) Effect of c-di-GMP synthase (PA5487) and degradation enzyme (PA3947) on engXCA expression. The genes for in trans expression were cloned under the control of the lac promoter in expression vector pDSK519 (pDSK); the empty vector was introduced into wild-type strain 8004 (WT) and its ΔrpfG deletion mutant as controls. (C) RT-PCR analysis of engXCA with the strains described above. The relative signal intensity for each strain was derived after normalization against the corresponding 16S rRNA loading control. In panels A and B, the data are the means of three repeats, and the error bars indicate standard deviation.