TABLE 1.
Strains and plasmids used in this study
| Strain or plasmida | Properties or characteristics | Source or reference |
|---|---|---|
| X. campestris pv. campestris strains | ||
| WT | Wild-type strain 8004, Rifr | Max Dow |
| FE58 | DSF reporter strain | 29 |
| ΔrpfF strain | rpfF in-frame deletion mutant of strain 8004 | 9 |
| Δclp strain | clp in-frame deletion mutant of strain 8004 | Present study |
| ΔrpfG strain | rpfG in-frame deletion mutant of strain 8004 | Present study |
| WT(pDSK) strain | Strain 8004 harboring plasmid pDSK519, Rifr, Kanr | Present study |
| WT(PA5487) strain | Strain 8004 harboring construct pDSK-PA5487, Rifr, Kanr | Present study |
| ΔrpfG(pDSK) strain | ΔrpfG mutant harboring plasmid pDSK519, Rifr, Kanr | Present study |
| ΔrpfG(PA3947) strain | ΔrpfG mutant harboring construct pDSK-PA3947, Rifr, Kanr | Present study |
| ΔrpfG(clp) strain | ΔrpfG mutant harboring construct pDSK-clp, Rifr, Kanr | Present study |
| Δclp[clp(E99S)] strain | Δclp mutant harboring construct pDSK-clp(E99S), Rifr, Kanr | Present study |
| Δclp[clp(T149S)] strain | Δclp mutant harboring construct pDSK-clp(T149S), Rifr, Kanr | Present study |
| Δclp(clp) strain | Δclp mutant harboring construct pDSK-clp, Rifr, Kanr | Present study |
| E99S strain | Strain 8004 derivative with clp replaced in situ by clp(E99S) | Present study |
| T149S strain | Strain 8004 derivative with clp replaced in situ by clp(T149S) | Present study |
| E99S(pDSK) strain | E99S mutant harboring plasmid pDSK519, Rifr, Kanr | Present study |
| E99S(PA5487) strain | E99S mutant harboring vector pDSK-PA5487, Rifr, Kanr | Present study |
| T149S(pDSK) strain | T149S mutant harboring plasmid pDSK519, Rifr, Kanr | Present study |
| T149S(PA5487) strain | T149S mutant harboring vector pDSK-PA5487, Rifr, Kanr | Present study |
| E. coli strains | ||
| DH5α | supE44 ΔlacU169(φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory collection |
| BL21 | F−ompT hsdSB(rB− mB−) gal dcm λ(DE3) | Laboratory collection |
| RK2013 | Triparental mating helper strain, Kanr | Laboratory collection |
| Plasmids | ||
| pDSK519 | Broad-host-range cloning vector, Kanr | Laboratory collection |
| pGEX-6P-1 | GST fusion protein expression vector, Ampr | H. W. Song |
| pGEM-4-GUS | Plasmid pGEM-4 containing promoterless gusA, Ampr | Present study |
| pLAFR3 | Broad-host-range cloning vector, Tecr | Laboratory collection |
| pGEMT-easy | Plasmid vector for cloning PCR fragment, Ampr | Promega |
| pK18mobsacB | Sucrose-sensitive suicide plasmid, Kanr | Laboratory collection |
| pL3PengXCA-GUS | DSF sensor with gusA under the control of the engXCA promoter | 29 |
| pDSK-clp | clp placed under the control of the lac promoter in vector pDSK519, Kanr | Present study |
| pDSK-clp(E99S) | clp(E99S) placed under the control of the lac promoter in vector pDSK519, Kanr | Present study |
| pDSK-clp(T149S) | clp(T149S) placed under the control of the lac promoter in vector pDSK519, Kanr | Present study |
| pDSK-PA3947 | PA3947 placed under the control of the lac promoter in vector pDSK519, Kanr | Present study |
| pDSK-PA5487 | PA5487 placed under the control of the lac promoter in vector pDSK519, Kanr | Present study |
| pGEM-clp | clp cloned in pGEMT-easy, Ampr | Present study |
| pGEX-clp | clp cloned in vector pGEX-6P-1 for protein purification, Ampr | Present study |
| pGEX-Smclp | clp homologue from Stenotrophomonas maltophilia cloned in vector pGEX-6P-1 for protein purification, Ampr | Present study |
| pGEX-clp(E99S) | clp(E99S) cloned in vector pGEX-6P-1 for protein purification, Ampr | Present study |
| pGEX-clp(T149S) | clp(T149S) cloned in vector pGEX-6P-1 for protein purification, Ampr | Present study |
| pK-clp(E99S) | clp(E99S) cloned in vector pK18mobsacB, Kanr | Present study |
| pK-clp(T149S) | clp(T149S) cloned in vector pK18mobsacB, Kanr | Present study |
a
WT, wild type.