TABLE 4.
Sensitivity of the LAMP assay using DNA template mixtures obtained from small amounts of bacterial cells
| No. of bacterial cells (CFU/LAMP tube) |
LAMP results (no. positive/no. tested) using a primer set(s) fora: |
|||||||
|---|---|---|---|---|---|---|---|---|
| AQ3815 | AQ4037 | AT4 | Vp02 | tdh | trh1 | trh2 | tdh, trh1, and trh2 | trh1 and trh2 |
| 6.7 | 170.4 | 40.0 | 105,000 | ND | + (3/3) | ND | ND | ND |
| 3.4 | 85.2 | 20.0 | 105,000 | ND | ± (2/3) | ND | ND | ND |
| 1.7 | 42.6 | 10.0 | 105,000 | + (3/3) | ± (2/3) | + (3/3) | ND | ND |
| 0.8 | 21.3 | 5.0 | 105,000 | ± (2/3) | − (0/3) | ± (2/3) | + (3/3) | + (3/3) |
a
+, triplicate assays were all positive. ±, triplicate assays were both positive and negative; −, triplicate assays were all negative; ND, not determined.