TABLE 2.
16S rRNA gene data for the strains studied
| Strain | 16S rRNA gene characteristics |
T-RF size (nt) |
GenBank accession no. | |||||
|---|---|---|---|---|---|---|---|---|
| Gene copy no. | WAVE-PCR fragmenta |
Sequence data |
Exptl datab |
|||||
| Size (bp) | G+C content (%) | AluI | HhaI | AluI | HhaI | |||
| Clostridium acetobutylicum ATCC 824 | 11 | 491 | 53.8 | 229 | 223 | 229.2 ± 0.1 | 223.1 ± 0.1 | NC003030 |
| Methylobacterium extorquens CM4 | 5 | 472 | 56.1 | 145 | 343 | 145.6 ± 0.1 | 344.0 ± 0.1 | NC011757 |
| Pseudomonas chlororaphis subsp. chlororaphis DSM 50083 | 4-7c | 521 | 53.6 | 72 | 207 | 72.0 ± 0.1 | 207.3 ± 0.1 | EF620458 |
| Pseudomonas stutzeri KC | 4-7c | 521 | 53.2 | 72 | 207 | 72.0 ± 0.1 | 207.3 ± 0.1 | AF063219 |
| Staphylococcus aureus subsp. aureus ATCC 25904 | 5 | 534 | 51.1 | 74 | 238 | 73.6 ± 0.2 | 238.7 ± 0.1 | NC009641 |
a
Fragment obtained with PCR primers 27f and 534r.
b
Data obtained with 6-FAM-labeled PCR primer 27f, primer 926r, and 6-FAM-labeled size standard.
c
Estimated from data for other sequenced Pseudomonas strains (accession no. NC009646, NC004129, NC002947, NC009434, NC007005, NC008027, and NC009439).