Figure 1.

Secondary structure prediction and function of SynToc75. (A) Toc75 from pea and SynToc75 are predicted to form multiple transmembrane β-sheets (13). (B) Heterologously expressed SynToc75 was recovered from inclusion bodies (lane 1) and was purified further by anion exchange chromatography (lane 2). A Coomassie brilliant blue-stained SDS/PAGE is shown. In lanes 3 is an immunoblot of purified SynToc75. Lanes: 1 and 2, 10 μg of protein; 3, 1 μg of protein. (C) Current trace from a bilayer containing eight active SynToc75 channels after a voltage step from 0 to −80 mV with 250 mM KCl, 10 mM CaCl₂, and 10 mM Hepes⋅Tris (pH 7.2) on both sides of the membrane.