Immunogold labeling of ultra-thin sections of Synechocystis PCC6803 by using αSynToc75. Cells were fixed with 0.5% paraformaldehyd and were embedded in LRWhite by using standard methods. Colloidal gold ( 5 nm) coupled to the secondary antibody was used for visualization. The position of gold grains is indicated by arrowheads. [Bars = 200 nm (A), and 100 nm (B–D).] (A, ×70,000; B–D, ×125,000.)