TABLE 1.
Primers used for the amplification of genes prior to subcloning into pVJGFP series plasmids
| Proteina | Direction | Sequence (5′-3′)b |
|---|---|---|
| CCP2 | Forward | GCGCGCTAGCTGTGGGCAGCCCCGAAAC |
| CCP2 | Reverse | GCGCGGATCCTTAGCACCGAGGAATCTTCTC |
| CCP1-CCP2 | Forward | GCGCGCTAGCTGCCCCCAGCCCAAGAC |
| CCP1-CCP2 | Reverse | Same as the CCP2 reverse primer |
| IFN-α | Forward | CGCGGCTAGCATGGCCTTGACCTTTGC |
| IFN-α | Reverse | CGCGGGATCCTCATTCCTTACTTCTTAAACTTTC |
| XAcat | Forward | CGCGGCTAGCATGATACCTGCTCTGAAAGAAG |
| XAcat | Reverse | GCGCGGATCCCTACTCAGGTGCCACTATCG |
| MBP | Forward | GCGCGCTAGCGAAGAAGGTAAACTGGTAATCTG |
| MBP | Reverse | GCGCCTCGAGTCAAGTCTGCGCGTCTTTCAGGG |
a
CCP2, human C1r CCP2 domain; CCP1-CCP2, human C1r CCP1-CCP2 domain pair; IFN-α, human IFN-α2b; GFP; A. victoria GFP; XAcat, T. maritima xylanase A catalytic domain; MBP, E. coli MBP.
b
The parts of the sequences corresponding to coding regions are indicated in boldface, and restriction sites are underlined. The NheI and BamHI restriction sites were used for all genes, except for MBP, where NheI and XhoI sites were used.