FIG. 1.

Yeast growth and zymogram showing the Bdh1p activity of the FY834 strain extracts at different times under respirofermentative conditions. (A) Growth curve of the FY834 yeast strain (in rich medium at 2% glucose) under respirofermentative conditions superimposed on the glucose concentrations of the supernatants. (B) Zymogram of the yeast extracts prepared at the times indicated in the growth curve. Sample G5 was taken at an OD of 34. Bdh1p bands on the gel were visualized by activity staining with (2R,3R)-2,3-butanediol and NAD.