TABLE 1.
Resistance to and uptake of tellurite in the mutant strain carrying the plasmids used in this work
| Strain | Tellurite resistance (OD660)a | Tellurite uptake (% of wild type)b |
|---|---|---|
| B100-5 | NG | 100 |
| RTE36-5 | 1.126 ± 0.047 | 22.1 ± 3.3 |
| RTE36-32 (actP) | NG | 113.7 ± 11.7 |
| RTE36-40 (acs + actP) | NG | 142.84 ± 23.6 |
| RTE36-42 (acs) | 1.083 ± 0.018 | 19.08 ± 1.34 |
| RTE36-320 (actP) | NG | 126.0 ± 11.6 |
a
Resistance was measured as optical density at 660 nm (OD660) reached by liquid cultures after 48 h, in the presence of 50 μM TeO32−. NG, no growth.
b
Tellurite uptake was measured in the presence of 100 μM TeO32−. The wild-type uptake rate, corresponding to 100%, is 4.274 ± 0.203 nmol/mg protein/min.