TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference |
|---|---|---|
| B. subtilis | ||
| WH557 | trpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 | 9 |
| WH557-gfp | trpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 amyE::Pxyl/tet-gfpmut2 | 9 |
| WH558 | trpC2 lacA::Pt17-tetR lox66-aphAIII-lox71 amyE::Pxyl/tet-lacZ | 9 |
| E. coli | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 26 |
| XL1-Blue | hsdR17(rK− mK+) recA1 endA1 gyrA96 thi-1 supE44 relA1 lac [F′ proAB lacIq ZΔM15 Tn10 (Tetr)] | Stratagene |
| S. aureus | ||
| SA113 (ATCC 35556) | NCTC8325 derivative; agr negative; 11-bp deletion in rbsU | 29 |
| RN4220 | NCTC8325-4 derivative; acceptor of foreign DNA | 29 |
| RAB133 | arcABDCR::lox72 | 40 |
| RAB171 | SA113 fdh::Pxyl/tet-gfpmut2 | This work |
| RAB180 | RN4220 lip::Pt17-tetR lox66-aphAIII-lox71 | This work |
| RAB190 | RN4220 lip::Pt17-tetR lox72 | This work |
| RAB200 | SA113 lip::Pt17-tetR lox66-aphAIII-lox71 | This work |
| RAB210 | SA113 lip::Pt17-tetR lox72 | This work |
| RAB211 | SA113 lip::Pt17-tetR lox72 fdh::Pxyl/tet-gfpmut2 | This work |
| RAB215 | SA113 lip::Pt17-tetR lox66-aphAIII-lox71 Pxyl/tet-zwf | This work |
| RAB216 | SA113 lip::Pt17-tetR lox72 Pxyl/tet-zwf | This work |
| RAB220 | SA113 lip::Pt17-revtetR lox66-aphAIII-lox71 | This work |
| RAB230 | SA113 lip::Pt17-revtetR lox72 | This work |
| RAB231 | SA113 lip::Pt17-revtetR lox72 fdh::Pxyl/tet-gfpmut2 | This work |
| RAB235 | SA113 lip::Pt17-revtetR lox66-aphAIII-lox71 Pxyl/tet-zwf | This work |
| RAB236 | SA113 lip::Pt17-revtetR-r2 lox72 Pxyl/tet-zwf | This work |
| S. carnosus | ||
| TM300 | Wild type | 50 |
| Plasmids | ||
| pBT2 | cat bla; E. coli/Staphylococcus shuttle vector; thermosensitive ori for staphylococci | 10 |
| pCX19 | cat xylR lip; Staphylococcus vector | 28 |
| pIC156 | Source of specR | 52 |
| pRAB1 | cat bla PpagA-cre; pBT2 derivative | 40 |
| pRAB2(-r2) | cat bla ′lip′-Pt17-(rev-)tetR-lox66-aphAIII-lox72-′lip′; pBT2 derivative | This work |
| pRAB4 | cat bla ′SAOUHSC_00139-141-Pxyl/tet-gfpmut2-specR-SAOUHSC_00143′; pBT2 derivative | This work |
| pRAB6 | cat bla ble Pxyl/tet-AS ′fabI′; pRB473 derivative | This work |
| pRAB7(-r2) | cat Pt17-(rev-)tetR; pCX19 derivative | This work |
| pRAB8 | cat PR*-tetR Pxyl/tet-arcABDCR; pCX19 derivative | This work |
| pRAB9 | cat bla SAOUHSC_01599-lox66-aphAIII-lox72-Pxyl/tet-zwf; pBT2 derivative | This work |
| pRB473 | cat bla ble; E. coli/Staphylococcus shuttle vector | 11 |
| pWH105-gfpmut2 | bla ′amyE′-cat-Pxyl/tet-gfpmut2-′amyE′; pWH105 derivative | This work |
| pWH1925-r2 | Source of revtetR | 51 |
| pWH1935-1a | Source of tetR | 9 |
| pWH1935-2 | Source of Pxyl/tet | 9 |