FIG. 2.

Analysis of the CB gene and peptide in the transgenic tomato. (A and B) PCR analysis of the CB gene in wild-type and transgenic tomatoes. Genomic DNA isolated from 10 mg of fresh leaves from transgenic tomatoes (T₀ plants, panel A, lanes 4 to 6; T₁ plants, panel B, lanes 4 to 14) and nontransgenic control plants (A, lane 3, and B, lane 15) were used as templates for PCRs. The PCR products were analyzed on 1% (wt/vol) agarose gels. (A and B) Lanes 1, no template (negative control); lanes 2, PCR product amplified from plasmid pBI121-spCB (positive control); lane 3 in panel B is the PCR product amplified from plasmid pBI121 without the CB gene (negative control). (C) Analysis of peptide CB expressed in transgenic tomatoes. The levels of CB peptide in transgenic tomato plants were determined by Western hybridization analysis. Soluble protein extracts from 50 mg of fresh leaves of transgenic tomatoes (T₀ plant, lane 2; T₁ plants, lanes 4 through 7) and nontransgenic control plants (wild type [WT], lanes 1 and 3) were Western hybridized using a polyclonal antibody raised against CB. Lane 8 is a positive control with 0.05 μg of synthetic CB peptide.