Table 1.
Group names | Cells | Scaffolds | Media | Duration |
---|---|---|---|---|
Acellular | No cells | PCL/ECM | With dexamethasone (+media) | 4 days 8 days 16 days |
PCL MSCs+ | 250,000 MSCs | PCL | With dexamethasone (+media) | 4 days 8 days 16 days |
PCL coculture+ | 250,000 MSCs 250,000 marrow cells | PCL | With dexamethasone (+media) | 4 days 8 days 16 days |
PCL/ECM MSCs+ | 250,000 MSCs | PCL/ECM | With dexamethasone (+media) | 4 days 8 days 16 days |
PCL/ECM coculture+ | 250,000 MSCs 250,000 marrow cells | PCL/ECM | With dexamethasone (−media) | 4 days 8 days 16 days |
PCL MSCs− | 250,000 MSCs | PCL | Without dexamethasone (−media) | 4 days 8 days 16 days |
PCL coculture− | 250,000 MSCs 250,000 marrow cells | PCL | Without dexamethasone (−media) | 4 days 8 days 16 days |
PCL/ECM MSCs− | 250,000 MSCs | PCL/ECM | Without dexamethasone (−media) | 4 days 8 days 16 days |
PCL/ECM coculture− | 250,000 MSCs 250,000 marrow cells | PCL/ECM | Without dexamethasone (−media) | 4 days 8 days 16 days |
The acellular group was taken as the control. Each of the other groups represents one combination of the four factors: cells seeded, scaffold type, media composition, and duration.
PCL, poly(ɛ-caprolactone); MSC, mesenchymal stem cell; ECM, extracellular matrix.