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. Author manuscript; available in PMC: 2010 Mar 3.

Published in final edited form as: Mol Cancer Ther. 2009 Mar 3;8(3):571. doi: 10.1158/1535-7163.MCT-08-0811

NSC 676914 inhibits TPA-induction of NF-κB dependent genes. A, IL-6 mRNA expression. Detection by quantitative RT-PCR showed TPA induced IL-6 mRNA was repressed by NSC 676914 pretreatment. Total RNA was isolated and analyzed from 18 hours TPA-treated HEK293 cells pretreated with NSC 676914 or DMSO for 1 hour. Human IL-6 mRNA was normalized by GAPDH. B, Luciferase reporter assays of NF-κB targets. Promoter directed luciferase reporters for COX-2, uPAR, and VEGF were transiently transfected into HEK293 cells separately. The cells were pretreated with NSC 676914 followed by exposure to TPA (10 ng/ml) or DMSO for 18 hours. The luciferase activity attained by TPA induction only was set at 100% and used to compare the activity in the presence of NSC 676914. Assays were performed in triplicate.

NSC 676914 inhibits TPA-induction of NF-κB dependent genes. A, IL-6 mRNA expression. Detection by quantitative RT-PCR showed TPA induced IL-6 mRNA was repressed by NSC 676914 pretreatment. Total RNA was isolated and analyzed from 18 hours TPA-treated HEK293 cells pretreated with NSC 676914 or DMSO for 1 hour. Human IL-6 mRNA was normalized by GAPDH. B, Luciferase reporter assays of NF-κB targets. Promoter directed luciferase reporters for COX-2, uPAR, and VEGF were transiently transfected into HEK293 cells separately. The cells were pretreated with NSC 676914 followed by exposure to TPA (10 ng/ml) or DMSO for 18 hours. The luciferase activity attained by TPA induction only was set at 100% and used to compare the activity in the presence of NSC 676914. Assays were performed in triplicate.