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. Author manuscript; available in PMC: 2010 Mar 3.

Published in final edited form as: Mol Cancer Ther. 2009 Mar 3;8(3):571. doi: 10.1158/1535-7163.MCT-08-0811

NSC 676914 inhibits TPA induced and basal invasion of breast cancer cells and transformation of JB6 cells. A, A matrigel invasion assay in breast cancer cells. Three human breast cancer cell lines, MDA-MD-231, T47D, and MCF-7 were distributed in BD Biocoat matrigel invasion chamber and invasion activity was stimulated by TPA (10 ng/ml) for 48 hours after pretreatment with NSC 676914 or DMSO for 1h. Only invasive cells can digest the matrix and move through the chambers. (Values, relative ratio compared to vehicle.) ** The MDA-MB-231 cell line was tested with NSC 676914 (17 µM) alone without TPA induction. B, Anchorage-independent transformation response. JB6 cells were suspended in soft agar and incubated with medium containing TPA (10 ng/ml) with NSC 676914 or DMSO for 10 days.

NSC 676914 inhibits TPA induced and basal invasion of breast cancer cells and transformation of JB6 cells. A, A matrigel invasion assay in breast cancer cells. Three human breast cancer cell lines, MDA-MD-231, T47D, and MCF-7 were distributed in BD Biocoat matrigel invasion chamber and invasion activity was stimulated by TPA (10 ng/ml) for 48 hours after pretreatment with NSC 676914 or DMSO for 1h. Only invasive cells can digest the matrix and move through the chambers. (Values, relative ratio compared to vehicle.) ** The MDA-MB-231 cell line was tested with NSC 676914 (17 µM) alone without TPA induction. B, Anchorage-independent transformation response. JB6 cells were suspended in soft agar and incubated with medium containing TPA (10 ng/ml) with NSC 676914 or DMSO for 10 days.