Table 1.
Characteristics of the 17 Plasmodium vivax microsatellite loci, and their primers in 69 P. vivax samples from central Vietnam
| Locus | Repeat sequence* | Primers (5¢®3¢) [5¢ fluorescent dye] | Size range, basepairs | No. alleles | He† (first episode) | Polyclonal samples/ locus, % | Average alleles/locus |
|---|---|---|---|---|---|---|---|
| Pv6727 | (AGA)19 | F:[PET]-TTAGATGACCAGCCGCTTCAGG | 184–199 | 6 | 0.55 (0.54) | 21 | 1.2 |
| R:CCATCAATGTCCCGCTTAGCACC | |||||||
| Pv6635 | (GGA)4TGG(GGA)18 | F:[NED]-CGTTGACGAGGCTCTCCAGG | 164–194 | 11 | 0.85 (0.86) | 86 | 2 |
| R:CGTGTTGTGTGTGTCCCTTCAGC | |||||||
| Pvsal1814 | (AGA)44 | F:[6FAM]-AAACAGGCATTAGGTTTAAGAGTG | 515–677 | 41 | 0.96 (0.96) | 87 | 2.8 |
| R:CAGTGGCTTCTTCTTTAGTGG | |||||||
| MS1 | Karunaweera et al.9 | Karunaweera et al.9 | 214–241 | 11 | 0.85 (0.82) | 51 | 1.7 |
| MS2 | 170–315 | 32 | 0.96 (0.96) | 53 | 1.9 | ||
| MS3 | 182–200 | 9 | 0.76 (0.77) | 7 | 1.1 | ||
| MS4 | 185–266 | 17 | 0.87 (0.86) | 57 | 1.6 | ||
| MS5 | 157–291 | 17 | 0.92 (0.92) | 71 | 2 | ||
| MS6 | 157–260 | 19 | 0.91 (0.89) | 78 | 2.2 | ||
| MS7 | 138–212 | 11 | 0.83 (0.87) | 16 | 1.3 | ||
| MS8 | 201–304 | 24 | 0.95 (0.95) | 47 | 1.6 | ||
| MS9 | 139–182 | 18 | 0.88 (0.88) | 79 | 1.9 | ||
| MS10 | 184–225 | 14 | 0.90 (0.91) | 19 | 1 | ||
| MS12 | 206–235 | 9 | 0.78 (0.76) | 32 | 1.5 | ||
| MS15 | 203–297 | 21 | 0.91 (0.91) | 70 | 2 | ||
| MS16 | 183–321 | 22 | 0.92 (0.94) | 12 | 1 | ||
| MS20 | 189–229 | 14 | 0.81 (0.87) | 80 | 1.9 |
*
The number of repeats is based on the Sal-1 strain.
†
He = expected heterozygosity calculated from the total sample population.