Table 3.
Monkeypox virus DNA extracted from blood samples from experimentally infected macaques quantitated on two pan-Orthopoxvirus diagnostic platforms*
| Blood samples | Real-time PCR | PCR/ESI-MS |
|---|---|---|
| Macaque 1, day 4 | 4.1 | 4.6 |
| Macaque 1, day 6 | 5.9 | 8.0 |
| Macaque 1, day 9† | 6.1 | 8.0 |
| Macaque 2, day 4 | 5.0 | 4.5 |
| Macaque 2, day 6 | 5.2 | 5.2 |
| Macaque 2, day 8 | 6.0 | 8.2 |
| Macaque 3, day 4 | 3.4 | 4.2 |
| Macaque 3, day 6 | 4.5 | 4.9 |
| Macaque 3, day 8 | 5.1 | 5.3 |
*
Values are mean log10 genome equivalents per milliliter. PCR/ESI-MS = polymerase chain reaction coupled with electrospray ionization mass spectrometry.
†
This macaque was moribund and was killed on day 9.