Table 4.
MPXV and VACV DNAs extracted from spiked macaque blood containing the same concentration (sample 1) or different concentrations of each virus (sample 2 and 3) and quantitated on the pan-Orthopoxvirus platform*
| Macaque blood sample | 1 × 106 PFU/mL | PCR/ESI-MS | 1 × 107 PFU/mL | PCR/ESI-MS |
|---|---|---|---|---|
| 1 | NA | NA | MPXV, VACV | 3.5, 3.4 |
| 2 | VACV | 3.0 | MPXV | 3.6 |
| 3 | MPXV | 3.3 | VACV | 3.8 |
*
Values are mean log10 genome equivalents per milliliter. MPXV = monkeypox virus; VACV = vaccinia virus; PFU = plaque-forming units; PCR/ESI-MS = polymerase chain reaction coupled with electrospray ionization mass spectrometry; NA = not available.