Skip to main content
. 2010 Jan 29;5(1):e8809. doi: 10.1371/journal.pone.0008809

Figure 3. Staining of DCX mRNA by hybridization with an alkaline phosphatase labeled anti-sense cRNA probe in the hippocampus at different ages (non-radioactive in situ hybridization).

Figure 3

A, B, From birth to adulthood (A: 1 day, B: 28 years of age), hippocampal DCX labeling reveals a distinct staining pattern in the granule cell layer and hippocampus proper. C, In most granule cells (neonatal DG), the mRNA signal occurs outside the nucleus in a small cytoplasmic rim. D, Higher magnification of the DG area marked in (B). E, Application of a labelled sense cRNA probe to a serial section of (B). The absence of sense-probe labelling confirmed the specificity of the detection system for DCX-mRNA/cRNA antisense probe hybrids. Differential Interference Contrast. Scale bars A, B, 1 mm; C-E, 25 µm.