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. 2010 Jan 29;5(1):e8524. doi: 10.1371/journal.pone.0008524

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Cushion et al.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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P. carinii from the lungs of immunosuppressed rats were treated with 60 µg/ml anidulafungin for 24 hrs and reacted with a mAB to β-1,3-D-glucan conjugated to Alexafluor 594. Panel A: Non-anidulafungin treated P. carinii viewed by phase microsocopy showing a cluster of trophs (arrow) attached to a cyst; Panel B: the same cluster viewed by fluorescent microscopy; no trophs were stained by the antibody; Panel C: Anidulafungin-treated P. carinii viewed by phase contrast microscopy; and Panel D: the same cluster under fluorescent excitation. Note the punctate staining pattern in both samples. All panels magnified according to the bar in Panel C.