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. 2010 Jan 29;5(1):e8965. doi: 10.1371/journal.pone.0008965

Figure 3. Southern blot detection of the pMOD700 transposon CAT gene in DNA of the Rmontanensis700 transformant.

Figure 3

Lane 1: contained a 1 kbp-DNA marker ladder with relative migration positions indicated at left. Lane 2: 10 picograms of pMOD700 plasmid digested with XhoI. Lane 3: 100 nanograms of untransformed R. montanensis genomic DNA digested with HindIII. Lanes 4, 5 and 6 contained 100 nanograms of transformant Rmontanensis700 DNA digested with HindIII, PstI and MunI, respectively. The blot was hybridized with a CAT gene probe.