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. 2010 Jan 29;5(1):e8965. doi: 10.1371/journal.pone.0008965

Figure 5. Detection of GFPuv and CAT mRNAs in Rmontanensis700 RNA extracts by Northern blotting.

Figure 5

(A) Rickettsial RNA extracts electrophoresed on 1% agarose formaldehyde gels stained with EtBr. Lanes 1 and 4: 0.5 to 4.0 kb RNA marker ladder with sizes indicated at left. Lanes 2 and 5: untransformed R. montanensis RNA (5 µg). Lanes 3 and 6: Rmontanensis700 RNA (5 µg). (B) Northern blots of gels shown in panel A. Lanes 1–3 were hybridized with a GFPuv gene probe and lanes 4–6 with a CAT gene probe.