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. 2010 Jan 29;5(1):e8955. doi: 10.1371/journal.pone.0008955

Figure 4. TWEAK induces the expression of NFκB2 targets, CCL21 and CCL19, in tubular cells.

Figure 4

A) Time-dependent increase in CCL21a, CCL19 and RANTES mRNA expression in tubular cells treated with 100 ng/mL TWEAK or 30 ng/ml TNFα. Real time RT-PCR. Mean ±SEM of four independent experiments. *p<0.04 vs control. B) Time-dependent increase in CCL21 protein expression in tubular cells treated with 100 ng/mL TWEAK or 30 ng/mL TNFα. Mean ±SEM of four independent experiments. *p<0.03 vs control. C) Parthenolide (PTN) does not prevent TWEAK-induced CCL21a mRNA up-regulation at 24 h. Real time RT-PCR. Mean ±SEM of four experiments. *p<0.04 vs control; #p<0.04 vs TWEAK alone. D) Lactacystin prevents TWEAK-induced CCL21a mRNA up-regulation at 24 h. Real time RT-PCR. Mean ±SEM of four experiments. *p<0.04 vs control; #p<0.03 vs TWEAK alone.