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. 2010 Jan 29;5(1):e8955. doi: 10.1371/journal.pone.0008955

Figure 9. Absence or antagonism of TWEAK decreases CCL21 in AKI.

Figure 9

A) TWEAK deficiency or anti-TWEAK antibodies decrease kidney CCL21a mRNA in AKI at 24 h following injury. Real time RT-PCR. Mean ±SEM of 6/7 animals per group. *p<0.02 vs control, #p<0.05 vs AKI, †p<0.05 vs AKI + isotype IgG. B) Total kidney CCL21 protein expression in AKI at 24 h following renal injury. Measured by Western blot. Mean ± SEM of 6/7 animals per group. *p<0.03 vs control, #p<0.05 vs AKI, †p<0.05 vs AKI + sotype IgG. Representative Western blot showing CCL21 protein expression in AKI model. C) CCL21 immunohistochemistry. Note epithelial cell localization of increased CCL21 expression in mice with AKI, when compared with healthy control or mice with AKI treated with anti-TWEAK. Original magnification ×200. Detail ×400. Controls for the technique are stained with non-specific IgG. D) Quantification of CCL21 as mean ± SEM of 6/7 animals per group. *p<0.004 vs control, #p<0.002 vs AKI.