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. 2010 Jan 29;5(1):e8970. doi: 10.1371/journal.pone.0008970

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Dong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Extracts equivalent to ten embryos from non-irradiated (control) or irradiated (γ-IR) samples collected at MBT (st.8, A) and 4 h after the MBT (B) were incubated with radiolabeled cyclin A2 as described in the “Materials and Methods” section. Aliquots were removed at the indicated times and analyzed for cyclin A2 cleavage by SDS-PAGE and autoradiography. Control samples correspond to non-irradiated embryos. Arrows on the right denote radiolabeled Xenopus cyclin A2 (XA2) and its cleaved form.