Figure 3.

RPM1∷MYC is degraded after inoculation with avirulent P. syringae isolates, which trigger LZ-NBS-LRR resistance genes. (A) Each panel represents infection of RPM1∷MYC transgenics with P. syringae DC3000 expressing a particular avirulence gene or not (thin line) listed at left. Tissue was harvested at the time, in hpi listed at the top, and total protein blots were probed with either anti-c-Myc monoclonal or (B) marker proteins for various subcellular compartments listed at the bottom of each series of blots in B. Extract from control plants (vector) are included for anti-c-Myc and anti-BiP experiments. The arrow marks RPM1∷MYC in A. Star (∗) represent earliest time point where visible RPM1-dependent HR was observed, and ampersand (@) represents the earliest time point where RPS2-dependent HR was observed. This set of data is all from one set of extracts and the experiment was repeated three times.