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Titration of ribozyme with CYT-18. EΔP5abc ribozyme (100 nM) was pre-folded and bound to trace S*, and various concentrations of CYT-18 were added. After a 15 min incubation, the fraction of active ribozyme was determined by measuring the fraction of S* cleaved rapidly upon addition of guanosine. Full activity was observed with ~110 nM CYT-18, as indicated by the intersection of the rising and flat portions of the concentration dependence.
