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. Author manuscript; available in PMC: 2011 Jan 5.
Published in final edited form as: J Biomech. 2009 Oct 13;43(1):137. doi: 10.1016/j.jbiomech.2009.09.019

Figure 3.

Figure 3

Knockdown of MMP-2 gene expression in AF cells using RNA interference. Real-time RT-PCR (left) reveals greater than 95% knockdown of mRNA levels relative to a nonsense control for 2 out of the 5 shRNA constructs tested (F and G). An ELISA-based protein assay (right), however, shows effective sustained knockdown on the protein level for 4 out of the 5 constructs (E, F, G, and H).