Skip to main content
. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Oncogene. 2009 Nov 2;29(4):589–596. doi: 10.1038/onc.2009.366

Figure 1.

Figure 1

Notch3 regulates apoptosis through the modulation of pERK and Bcl-2 proteins in lung cancer cell line HCC2429. (A) Consistent with previous studies, Notch3 siRNA downregulates pERK expression after 48 hours of treatment with Notch3 siRNA. Expressions of pAkt, Foxo3a, PKA-Cα and PI3K are unchanged, suggesting that in lung cancer the MAPK pathway controls Notch3-dependent apoptosis. (B) Inhibition of Notch3 with siRNA upregulates pro-apoptotic Bax and downregulates expressions of the Bcl-2 pro-survival proteins pBcl-2, Bcl-xL and Mcl-1. (C) Among the BH3-only proteins, induction of Bim can be observed. By contrast, no change in the expression of Puma or Bid is seen. Note that pBad is upregulated in Notch3 siRNA treated cells compared to siRNA control. Similar to Bim, cytokines and growth factors regulate pBad, further supporting previous observations of significant crosstalk between Notch and EGFR pathway. (D) Compared to siRNA control, an increase of Bim mRNA was observed when lung cancer cell line HCC2429 was transfected with Notch3 siRNA, suggesting that Notch3 regulated Bim transcriptionally. In all siRNA experiments, non-silencing siRNA was used as control.