Table 2.
n | Activating IKs (pA/pF, +20 mV) | IKs tail (pA/pF) | V1/2 (mV) | τ-activation (ms, +20 mV) | τ-deactivation (ms, -40 mV) | |
---|---|---|---|---|---|---|
Control-1 | 7 | 25.2±5.1 | 18.9±45 | 23.9±2.6 | 1864.2±143.3 | 552.7±60.3 |
IAP54-56-IKs | 8 | 75.1±8.2 † | 36.4±6.1 † | 2.6±0.8 † | 987.4±127.5 † | 334.5±38.6 † |
Control-2 | 7 | 23.2±7.7 | 17.2±2.6 | 21.7±2.4 | 1859.8±125.7 | 564.5±53.2 |
WT NPPA fragment | 7 | 53.1±9.2 ‡ | 30.3±4.2 ‡ | 10.6±1.3 ‡ | 1448.8±106.4 * | 414.9±37.5 * |
Mutant NPPA S64R fragment | 7 | 77.9±9.7 ‡,∥ | 46.2±5.9 ‡,∥ | 0.8±1.1 ‡,∥ | 850.3±83.1 ‡,∥ | 307.7±30.5 ‡,§ |
Values are shown as mean±SEM; The activating IKs was elicited with 5-sec pulsing to +20 mV from a holding potential of -80 mV and the IKs tail was recorded upon pulsing back to -40 mV;
P<0.05 vs. control-2
P<0.001 vs. control-1
P<0.001 vs. control-2
P<0.05 vs. WT NPPA peptide fragment
P<0.001 vs. WT NPPA peptide fragment; there was no statistical difference between two control groups.