Table 1.
Galactosyltransferase activity in an enzyme extract derived from Haemonchus contortus adult worms
| Acceptor | Relative galactosyltransferase (GalT) activity |
|
|---|---|---|
| H. contortus GalT | Bovine α1,3-GalT | |
| GalNAcα-O-pNP | 100a | <1 |
| GalNAcβ-O-pNP | 13 | <1 |
| GalNAcβ1-4GlcNAc-R1b | 40 | 5 |
| Galβ1-4GlcNAc-pNP | <1 | 51 |
| Galβ-O-pNP | <1 | 41 |
| Galα-O-pNP | <1 | 4 |
| Fucα1-2Galβ1-3GlcNAc-R2 | <1 | 1 |
| Fucα1-2Galβ1-4GlcNAc-R1 | <1 | 2 |
| Galβ1-3GlcNAc-R1 | <1 | 100a |
All acceptor substrates in the assays have been used at a concentration of 1 mM. The acceptor specificity of the H. contortus enzyme extract has been compared with the activity of commercial bovine (α1,3-galactosyltransferase (α1,3-GalT). For both enzymes, the acceptor substrate that showed the highest activity has been set at 100%. In the assays with H. contortus extract, 100% activity represents an enzyme activity of 1 nmol/ml/h, and for the α1,3-GalT 100% activity represents an activity 49 nmol/ml/h.
a
Values set at 100%
b
R1 = -O-(CH2)8COOCH3; R2 = -(CH2)7CH3