Immune precipitates from vector alone, wild-type WRN, and c.611C>T WRN transfections were resolved by electrophoresis through an 8.5% polyacrylamide gel. The gel was transferred to PVDF, probed with a monoclonal anti-WRN antibody, and visualized on a Phosphorimager following ECL detection. Bands corresponding to WRN, and immunoglobulin heavy and light chains are indicated. Lane 1: (rWRN) purified recombinant WRN; lane 2: vector only control; lane 3: wild-type WRN; lane 4: P204L WRN. P204L WRN levels were 10-fold lower relative to wild-type WRN.