Table 1. Data collection, phasing and refinement statistics of the AP2 domain of PF14_0633 specifically bound to dsDNA.
| SeMet Anomalous Peak | Native | |
|---|---|---|
| Data Collection a | ||
| Space group | C2221 | C2221 |
| Cell dimensions a, b, c (Å) |
43.65, 59.18, 173.93 | 43.81, 58.82, 177.30 |
| Wavelength | 0.97886 Å | 0. 97886 Å |
| Resolution (Å) b | 50.0 – 2.40 (2.44–2.40) | 50.0–2.20 (2.26–2.20) |
| Rsym c | 0.107 (0.477) | 0.075 (0.381) |
| I / σI | 21.9 (2.5) | 32.1 (4.5) |
| Completeness (%) | 96.7 (71.3) | 91.9 (72.4) |
| Redundancy | 11.4 (4.2) | 11.0 (9.0) |
| Phasing | ||
| Figure of Merit (before/after solvent flattening) |
0.35/0.70 | |
| Refinement | ||
| Resolution (Å) | 29.55 – 2.20 (2.26–2.20) | |
| Rwork / Rfree | 0.230 / 0.279 | |
| No. atoms | 1691 | |
| Protein | 971 | |
| DNA | 623 | |
| Water | 97 | |
| <B-factors> | ||
| Protein | 59.1 | |
| DNA | 65.4 | |
| Water | 61.0 | |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.009 | |
| Bond angles (°) | 1.37 |
a
Data statistics from one crystal of Native or SeMet PF14_0633.
b
Values in parentheses are for highest-resolution shell.
c
Rsym = ΣΣj| Ij - <I> I/SIj, where Ij is the intensity measurement for reflection j and <I> is the mean intensity for multiply-recorded reflections.