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Surface immobilization strategies for single molecule FRET experiments of packaged Cy5-Cy5.5 labeled 5 kbp DNA or 50 kbp λ-DNA. HOC-IgY polyclonal antibodies bind procapsids to the support glass slide at the 155 HOC molecules decorating the capsid situated at the center of each capsomer. Individual immobilized double dye-labeled particles are then imaged following laser excitation as described.
